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Proteomics: Sequence, Structure, FunctionWorkshop I: High Throughput Technologies and Methods of AnalysisMarch 22 - 26, 2004Organizing Committee:
Tim Ting Chen, Chair
(University of Southern California)
IntroductionFocus: new technologies such as mass spectrometry and protein arrays and methods of analysis, both analysis of experiments and analysis leading to an understanding of protein networks. The workhorse of proteomics is 2-D gel electrophoresis. This technology spreads out proteins in a sample differentially in the plane, producing a collection of spots, each of which contains one protein. A comparatively new technology has come on line -- mass spectrometry -- that promises to be to proteomics what automated sequencing machines were to genomics. The basic modality is protein mass fingerprinting -- a protein is cut into segments and then the fragment masses measured very precisely using mass spec. By comparison with protein databases, the composition of the fragments can be determined, and from this the entire protein reconstructed. There are numerous challenges to making this an effective high-throughput method. Mass spec facilities are springing up at research facilities worldwide. In addition, there are a number of new technologies coming on line that will provide even more information. Each technology raises interesting issues of analysis, ranging from comparatively familiar issues of image analysis to the much more open issues of how to use the information generated to reconstruct networks of protein interactions. There are other new technologies that hold great promise -- for example, Protein Chips/microarrays, Yeast Two-hybrid Assays and Coimmunoprecipitation-2D Gel-Mass Spec -- that also will be included here. This workshop would include representatives from companies with products for mass spec and other high-throughput proteomics methods, and researchers who are users of these technologies and those engaged in the analysis of data generated by these technologies. Other links and events
SpeakersRuedi Aebersold (Institute for Systems Biology)Vineet Bafna (University of California at San Diego) Christoph Borchers (University of North Carolina) Tim Ting Chen (University of Southern California) Nathan Edwards (Applied Biosystems) Peter Harrington (Ohio University) Alex Hartemink (Duke University) Joseph Loo (UCLA) Bin Ma (University of West Ontario) Edward Marcotte (University of Texas, Austin) Nouri Neamati (University of Southern California) William Noble (University of Washington) Pavel Pevzner (University of California at San Diego) Fernando Pineda (Johns Hopkins University) Benno Schwikowski (Institute for Systems Biology) Michael Snyder (Yale University) Terry Speed (University of California at Berkeley) Shankar Subramaniam (University of California at San Diego) Fengzhu Sun (University of Southern California) Tim Veenstra (National Cancer Institute) Vicki H. Wysocki (University of Arizona) Austin Yang (University of Southern California) Golan Yona (Cornell University) Weixiong Zhang (Washington University) Hongyu Zhao (Yale University) Contact Us:Institute for Pure and Applied Mathematics (IPAM) |
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