Mass Spectrometry-Based Methods for Protein Identification

Joseph Loo
Biochemistry & Chemistry

A proteomics-based approach has been practiced for many years, in fact prior to the time the term “proteomics” was coined, to monitor changes in protein expression. The primary readout of protein expression was based on analysis of proteins separated by polyacrylamide gel electrophoresis (PAGE). However, development of novel mass spectrometry (MS) methods has made the identification of PAGE-separated proteins much more amenable and has contributed greatly to expand the range of applications to which proteomics can contribute. Provided the genome or protein sequences are known and available, MS provides a robust method for protein identification. The technology to automate the entire method of protein separation through to protein identification is available, thereby greatly increasing the throughput and overall capacity of the analysis.

Presentation (PowerPoint File)

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