The term "proteome" has been coined to reflect the revolutionary changes the field of biochemistry has been undergoing . This word refers to the PROTEins expressed by a genOME or tissue. Unlike the genome, the proteome is affected by a number of factors such as tissue and environmental conditions. A gene can be spliced in many different ways and proteins can be altered after translation. Hence, the proteome consists of far more proteins than the genome contains genes. Two-imensional polyacrylamide gel electrophoresis is currently the technique of choice to separate and display all the proteins expressed in a tissue. In the resulting protein map, individual protein spots are identified and quantified. To this end, background peaks are eliminated. We review the methodology implemented in the published literature to process the raw data and analyze the cleaned-up maps. We utilize wavelets to summarize the two-dimensional gels. Within this framework, we seek to compare different gels by determining the strength and the location of the signal.
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