Multidimensional fluorescence imaging

Paul French
Imperial College

This talk will review the development and application of multidimensional fluorescence imaging (MDFI), with an emphasis on fluorescence lifetime imaging (FLIM) optimised for high-speed imaging of live cells. FLIM combined with tunable excitation sources and spectral and polarisation-resolved imaging provides opportunities to distinguish different molecular species in a multidimensional space and to probe influence of the local fluorophore environment, including molecular interactions. FLIM and MDFI also provide opportunities for label-free molecular imaging in biological tissue.

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