Understanding the hierarchical organization of molecules, multi-protein assemblies, organelles and networks within the interior of a eukaryotic cell is a challenge of fundamental interest in cell biology. A wide variety of microscopic and spectroscopic methods already exist for imaging intact cells and their components: modern fluorescence microscopic methods provide powerful tools for imaging at spatial resolutions in the micron range, while emerging methods in electron microscopy can be used to image the arrangement of protein assemblies at resolutions of 1 nm or better. To take advantage of these rapid advances in imaging technology, it is critical to develop and apply advanced computational strategies for image processing that can cope both with the volume and complexity of the data. This conference seeks to bring together leaders at this interdisciplinary interface of image processing and stimulate new partnerships to address computational problems at this exciting frontier of cell biology. The one-week meeting will bring together biologists, physicists, mathematicians and specialists in microscopy and image analysis.